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Simple Simulation of Single-cell RNA Sequencing Data

Bioconductor version: Release (3.19)

Splatter is a package for the simulation of single-cell RNA sequencing count data. It provides a simple interface for creating complex simulations that are reproducible and well-documented. Parameters can be estimated from real data and functions are provided for comparing real and simulated datasets.

Author: Luke Zappia [aut, cre] (, lazappi), Belinda Phipson [aut] (, bphipson), Christina Azodi [ctb] (, azodichr), Alicia Oshlack [aut]

Maintainer: Luke Zappia <luke at>

Citation (from within R, enter citation("splatter")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

An introduction to the Splatter package HTML R Script
Splat simulation parameters HTML R Script
splatPop simulation HTML R Script
Reference Manual PDF


biocViews GeneExpression, ImmunoOncology, RNASeq, Sequencing, SingleCell, Software, Transcriptomics
Version 1.28.0
In Bioconductor since BioC 3.5 (R-3.4) (7 years)
License GPL-3 + file LICENSE
Depends R (>= 4.0), SingleCellExperiment
Imports BiocGenerics, BiocParallel, checkmate (>= 2.0.0), crayon, edgeR, fitdistrplus, grDevices, locfit, matrixStats, methods, rlang, S4Vectors, scuttle, stats, SummarizedExperiment, utils, withr
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Suggests BASiCS(>= 1.7.10), BiocManager, BiocSingular, BiocStyle, Biostrings, covr, cowplot, GenomeInfoDb, GenomicRanges, ggplot2 (>= 3.4.0), IRanges, igraph, knitr, limSolve, lme4, magick, mfa, phenopath, progress, preprocessCore, pscl, rmarkdown, scales, scater(>= 1.15.16), scDD, scran, SparseDC, spelling, testthat, VariantAnnotation, zinbwave
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Depends On Me
Imports Me SCRIP
Suggests Me mastR, NewWave, scone, scPCA, smartid, scellpam
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package splatter_1.28.0.tar.gz
Windows Binary
macOS Binary (x86_64) splatter_1.28.0.tgz
macOS Binary (arm64) splatter_1.28.0.tgz
Source Repository git clone
Source Repository (Developer Access) git clone
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Old Source Packages for BioC 3.19 Source Archive