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ATACseqQC

This is the released version of ATACseqQC; for the devel version, see ATACseqQC.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17, 3.16, 3.15, 3.14, 3.13, 3.12, 3.11, 3.10, 3.9, 3.8, 3.7, 3.6, 3.5

ATAC-seq Quality Control


Bioconductor version: Release (3.23)

ATAC-seq, an assay for Transposase-Accessible Chromatin using sequencing, is a rapid and sensitive method for chromatin accessibility analysis. It was developed as an alternative method to MNase-seq, FAIRE-seq and DNAse-seq. Comparing to the other methods, ATAC-seq requires less amount of the biological samples and time to process. In the process of analyzing several ATAC-seq dataset produced in our labs, we learned some of the unique aspects of the quality assessment for ATAC-seq data.To help users to quickly assess whether their ATAC-seq experiment is successful, we developed ATACseqQC package partially following the guideline published in Nature Method 2013 (Greenleaf et al.), including diagnostic plot of fragment size distribution, proportion of mitochondria reads, nucleosome positioning pattern, and CTCF or other Transcript Factor footprints.

Author: Jianhong Ou, Haibo Liu, Feng Yan, Jun Yu, Michelle Kelliher, Lucio Castilla, Chao Deng, Nathan Lawson, Lihua Julie Zhu

Maintainer: Jianhong Ou <jou at morgridge.org>

Citation (from within R, enter citation("ATACseqQC")):

Jianhong Ou, Haibo Liu, Feng Yan, Jun Yu, Michelle Kelliher, Lucio Castilla, Chao Deng, Nathan Lawson, Lihua Julie Zhu. ATACseqQC: ATAC-seq Quality Control. doi:10.18129/B9.bioc.ATACseqQC, R package version 1.36.1, https://bioconductor.org/packages/ATACseqQC.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("ATACseqQC")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("ATACseqQC")
ATACseqQC Vignette HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews ATACSeq, Coverage, DNASeq, GeneRegulation, ImmunoOncology, NucleosomePositioning, QualityControl, Sequencing, Software
Version1.36.1
In Bioconductor sinceBioC 3.5 (R-3.4) (9.5 years)
License GPL (>= 2)
Depends R (>= 3.5.0), BiocGenerics, S4Vectors
Imports BSgenome, Biostrings, ChIPpeakAnno, IRanges, GenomicRanges, GenomicAlignments, GenomeInfoDb, GenomicScores, graphics, grid, limma, polynom, Rsamtools (>= 1.31.2), randomForest, rtracklayer, stats, motifStack, utils, KernSmooth, edgeR, BiocParallel
System Requirements
URL
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Suggests BiocStyle, knitr, BSgenome.Hsapiens.UCSC.hg19, TxDb.Hsapiens.UCSC.hg19.knownGene, phastCons100way.UCSC.hg19, MotifDb, trackViewer, testthat, rmarkdown
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Depends On Me
Imports Me NADfinder
Suggests Me ATACseqTFEA
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package ATACseqQC_1.36.1.tar.gz
Windows Binary (x86_64) ATACseqQC_1.36.1.zip
macOS Binary (big-sur-x86_64) ATACseqQC_1.36.1.tgz
macOS Binary (sonoma-arm64) ATACseqQC_1.36.1.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/ATACseqQC
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/ATACseqQC
Package Short Url https://bioconductor.org/packages/ATACseqQC/
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Old Source Packages for BioC 3.23Source Archive