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Simulate RNA-seq reads

Bioconductor version: Release (3.18)

This package can be used to simulate RNA-seq reads from differential expression experiments with replicates. The reads can then be aligned and used to perform comparisons of methods for differential expression.

Author: Alyssa C. Frazee, Andrew E. Jaffe, Rory Kirchner, Jeffrey T. Leek

Maintainer: Jack Fu <jmfu at jhsph.edu>, Jeff Leek <jtleek at gmail.com>

Citation (from within R, enter citation("polyester")):


To install this package, start R (version "4.3") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

The Polyester package for simulating RNA-seq reads HTML R Script
Reference Manual PDF


biocViews DifferentialExpression, Sequencing, Software
Version 1.38.0
In Bioconductor since BioC 3.0 (R-3.1) (9.5 years)
License Artistic-2.0
Depends R (>= 3.0.0)
Imports Biostrings(>= 2.32.0), IRanges, S4Vectors, logspline, limma, zlibbioc
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package polyester_1.38.0.tar.gz
Windows Binary polyester_1.38.0.zip
macOS Binary (x86_64) polyester_1.38.0.tgz
macOS Binary (arm64) polyester_1.38.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/polyester
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/polyester
Bioc Package Browser https://code.bioconductor.org/browse/polyester/
Package Short Url https://bioconductor.org/packages/polyester/
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