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Data Mining for RNA-seq data: normalization, feature selection and classification

Bioconductor version: Release (3.19)

The DaMiRseq package offers a tidy pipeline of data mining procedures to identify transcriptional biomarkers and exploit them for both binary and multi-class classification purposes. The package accepts any kind of data presented as a table of raw counts and allows including both continous and factorial variables that occur with the experimental setting. A series of functions enable the user to clean up the data by filtering genomic features and samples, to adjust data by identifying and removing the unwanted source of variation (i.e. batches and confounding factors) and to select the best predictors for modeling. Finally, a "stacking" ensemble learning technique is applied to build a robust classification model. Every step includes a checkpoint that the user may exploit to assess the effects of data management by looking at diagnostic plots, such as clustering and heatmaps, RLE boxplots, MDS or correlation plot.

Author: Mattia Chiesa <mattia.chiesa at>, Luca Piacentini <luca.piacentini at>

Maintainer: Mattia Chiesa <mattia.chiesa at>

Citation (from within R, enter citation("DaMiRseq")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

Data Mining for RNA-seq data: normalization, features selection and classification - DaMiRseq package PDF R Script
Reference Manual PDF


biocViews Classification, ImmunoOncology, RNASeq, Sequencing, Software
Version 2.16.0
In Bioconductor since BioC 3.5 (R-3.4) (7 years)
License GPL (>= 2)
Depends R (>= 3.5.0), SummarizedExperiment, ggplot2
Imports DESeq2, limma, EDASeq, RColorBrewer, sva, Hmisc, pheatmap, FactoMineR, corrplot, randomForest, e1071, caret, MASS, lubridate, plsVarSel, kknn, FSelector, methods, stats, utils, graphics, grDevices, reshape2, ineq, arm, pls, RSNNS, edgeR, plyr
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Source Package DaMiRseq_2.16.0.tar.gz
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