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polyICSFlow

This is the development version of polyICSFlow; to use it, please install the devel version of Bioconductor.

Identifying the Frequency of Polyfunctional Antigen-Specific T cells in ICS Flow Cytometry Data


Bioconductor version: Development (3.24)

polyICSFlow systematically identifies all cytokine combinations detected in an intracellular cytokine staining (ICS) flow cytometry assay and quantifies polyfunctional antigen-specific responses to single or multiple antigens. The package also offers versatile plotting options for efficient data exploration and visualization of cytokine co-expression profiles.

Author: Lisa Loksø Dietz [aut, cre] ORCID iD ORCID: 0000-0002-1797-5766 , Sofie Van Gassen [aut] ORCID iD ORCID: 0000-0002-7119-5330 , Sarah Bonte [aut] ORCID iD ORCID: 0000-0002-0637-4893 , Yvan Saeys [aut] ORCID iD ORCID: 0000-0002-0415-1506 , Ole Schmeltz Søgaard [aut] ORCID iD ORCID: 0000-0001-9107-2023

Maintainer: Lisa Loksø Dietz <lisdie at clin.au.dk>

Citation (from within R, enter citation("polyICSFlow")):

Lisa Loksø Dietz, Sofie Van Gassen, Sarah Bonte, Yvan Saeys, Ole Schmeltz Søgaard. polyICSFlow: Identifying the Frequency of Polyfunctional Antigen-Specific T cells in ICS Flow Cytometry Data. doi:10.18129/B9.bioc.polyICSFlow, R package version 0.99.6, https://bioconductor.org/packages/polyICSFlow.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("polyICSFlow")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("polyICSFlow")
getting_started HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews FlowCytometry, Immunology, SingleCell, Software
Version0.99.6
In Bioconductor sinceBioC 3.24 (R-4.6)
License GPL (>=2)
Depends R (>= 3.5.0)
Imports flowCore, flowWorkspace, openCyto, FlowSOM, ComplexHeatmap, SummarizedExperiment, cowplot, dplyr, forcats, ggtext, rlang, scales, stringr, tidyselect, tidyr, magrittr, ggplot2, methods
System Requirements
URLhttps://github.com/SoegaardLab/polyICSFlow
Bug Reportshttps://github.com/SoegaardLab/polyICSFlow/issues
See More
Suggests knitr, rmarkdown, testthat (>= 3.0.0), patchwork, ggh4x
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package polyICSFlow_0.99.6.tar.gz
Windows Binary (x86_64) polyICSFlow_0.99.6.zip
macOS Binary (big-sur-x86_64) polyICSFlow_0.99.6.tgz
macOS Binary (sonoma-arm64) polyICSFlow_0.99.6.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/polyICSFlow
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/polyICSFlow
Package Short Url https://bioconductor.org/packages/polyICSFlow/
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