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Spillover Compensation in Mass Cytometry Data

Bioconductor version: Release (3.19)

Channel interference in mass cytometry can cause spillover and may result in miscounting of protein markers. We develop a nonparametric finite mixture model and use the mixture components to estimate the probability of spillover. We implement our method using expectation-maximization to fit the mixture model.

Author: Marco Guazzini [aut, cre] , Alexander G. Reisach [aut], Sebastian Weichwald [aut] , Christof Seiler [aut]

Maintainer: Marco Guazzini <m.guazzini at>

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biocViews FlowCytometry, ImmunoOncology, MassSpectrometry, Preprocessing, Regression, SingleCell, Software, StatisticalMethod, Visualization
Version 1.0.0
In Bioconductor since BioC 3.19 (R-4.4) (< 6 months)
License LGPL-3
Depends R (>= 4.3.0), SummarizedExperiment, CATALYST
Imports dplyr, tibble, tidyselect, stats, ggplot2, tidyr, spatstat.geom, S4Vectors, parallel
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