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Preprocessing of Illumina Infinium 450K data

Bioconductor version: Release (3.19)

Precise measurements are important for epigenome-wide studies investigating DNA methylation in whole blood samples, where effect sizes are expected to be small in magnitude. The 450K platform is often affected by batch effects and proper preprocessing is recommended. This package provides functions to read and normalize 450K '.idat' files. The normalization corrects for dye bias and biases related to signal intensity and methylation of probes using local regression. No adjustment for probe type bias is performed to avoid the trade-off of precision for accuracy of beta-values.

Author: Jonathan Alexander Heiss

Maintainer: Jonathan Alexander Heiss <jonathan.heiss at>

Citation (from within R, enter citation("normalize450K")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

Normalization of 450K data PDF R Script
Reference Manual PDF


biocViews DNAMethylation, MethylationArray, Microarray, Normalization, Preprocessing, Software, TwoChannel
Version 1.32.0
In Bioconductor since BioC 3.3 (R-3.3) (8 years)
License BSD_2_clause + file LICENSE
Depends R (>= 3.3), Biobase, illuminaio, quadprog
Imports utils
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Source Package normalize450K_1.32.0.tar.gz
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macOS Binary (x86_64) normalize450K_1.32.0.tgz
macOS Binary (arm64) normalize450K_1.32.0.tgz
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