Registration Open for Bioc2024 July 24-26


Iteratively Adjusted Surrogate Variable Analysis

Bioconductor version: Release (3.19)

Iteratively Adjusted Surrogate Variable Analysis (IA-SVA) is a statistical framework to uncover hidden sources of variation even when these sources are correlated. IA-SVA provides a flexible methodology to i) identify a hidden factor for unwanted heterogeneity while adjusting for all known factors; ii) test the significance of the putative hidden factor for explaining the unmodeled variation in the data; and iii), if significant, use the estimated factor as an additional known factor in the next iteration to uncover further hidden factors.

Author: Donghyung Lee [aut, cre], Anthony Cheng [aut], Nathan Lawlor [aut], Duygu Ucar [aut]

Maintainer: Donghyung Lee <Donghyung.Lee at>, Anthony Cheng <Anthony.Cheng at>

Citation (from within R, enter citation("iasva")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

Detecting hidden heterogeneity in single cell RNA-Seq data HTML R Script
Reference Manual PDF


biocViews BatchEffect, FeatureExtraction, ImmunoOncology, Preprocessing, QualityControl, RNASeq, Software, StatisticalMethod
Version 1.22.0
In Bioconductor since BioC 3.8 (R-3.5) (5.5 years)
License GPL-2
Depends R (>= 3.5)
Imports irlba, stats, cluster, graphics, SummarizedExperiment, BiocParallel
System Requirements
See More
Suggests knitr, testthat, rmarkdown, sva, Rtsne, pheatmap, corrplot, DescTools, RColorBrewer
Linking To
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report Build Report

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package iasva_1.22.0.tar.gz
Windows Binary
macOS Binary (x86_64) iasva_1.22.0.tgz
macOS Binary (arm64) iasva_1.22.0.tgz
Source Repository git clone
Source Repository (Developer Access) git clone
Bioc Package Browser
Package Short Url
Package Downloads Report Download Stats