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Positive Droplet Calling for DNA Methylation Droplet Digital PCR

Bioconductor version: Release (3.19)

Reads files exported from 'QX Manager or QuantaSoft' containing amplitude values from a run of ddPCR (96 well plate) and robustly sets thresholds to determine positive droplets for each channel of each individual well. Concentration and normalized concentration in addition to other metrics is then calculated for each well. Results are returned as a table, optionally written to file, as well as optional plots (scatterplot and histogram) for both channels per well written to file. The package includes a shiny application which provides an interactive and user-friendly interface to the full functionality of PoDCall.

Author: Hans Petter Brodal [aut, cre], Marine Jeanmougin [aut], Guro Elisabeth Lind [aut]

Maintainer: Hans Petter Brodal <hans.petter.brodal at>

Citation (from within R, enter citation("PoDCall")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

PoDCall: Positive Droplet Caller for DNA Methylation ddPCR HTML R Script
Reference Manual PDF


biocViews Classification, CpGIsland, DNAMethylation, DifferentialMethylation, Epigenetics, Software, ddPCR
Version 1.12.0
In Bioconductor since BioC 3.13 (R-4.1) (3 years)
License GPL-3
Depends R (>= 4.4)
Imports ggplot2, gridExtra, mclust, diptest, rlist, shiny, DT, LaplacesDemon, purrr, shinyjs, readr, grDevices, stats, utils
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Suggests knitr, rmarkdown, testthat, BiocStyle
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Follow Installation instructions to use this package in your R session.

Source Package PoDCall_1.12.0.tar.gz
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macOS Binary (x86_64) PoDCall_1.12.0.tgz
macOS Binary (arm64) PoDCall_1.12.0.tgz
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Source Repository (Developer Access) git clone
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