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DNABarcodeCompatibility

A Tool for Optimizing Combinations of DNA Barcodes Used in Multiplexed Experiments on Next Generation Sequencing Platforms

Bioconductor version: 3.23 · Package version: 1.28.0

Other Bioconductor versions

devel is the development version; release is the current stable one.

3.24 (devel), 3.23 (release)

The package allows one to obtain optimised combinations of DNA barcodes to be used for multiplex sequencing. In each barcode combination, barcodes are pooled with respect to Illumina chemistry constraints. Combinations can be filtered to keep those that are robust against substitution and insertion/deletion errors thereby facilitating the demultiplexing step. In addition, the package provides an optimiser function to further favor the selection of barcode combinations with least heterogeneity in barcode usage.

DOI: 10.18129/B9.bioc.DNABarcodeCompatibility

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("DNABarcodeCompatibility")

Details

MaintainerCéline Trébeau <ctrebeau@pasteur.fr>
AuthorCéline Trébeau [cre] (ORCID: <https://orcid.org/0000-0001-6795-5379>), Jacques Boutet de Monvel [aut] (ORCID: <https://orcid.org/0000-0001-6182-3527>), Fabienne Wong Jun Tai [ctb], Raphaël Etournay [aut] (ORCID: <https://orcid.org/0000-0002-2441-9274>)
Licensefile LICENSE
URLhttps://dnabarcodecompatibility.pasteur.fr/
Bug Reportshttps://gitlab.pasteur.fr/ida-public/dnabarcodecompatibility/-/issues
Source branchRELEASE_3_23
Build report Bioconductor build system, r-universe
biocViewsPreprocessing, Sequencing, Software
Package Short Url https://bioconductor.org/packages/DNABarcodeCompatibility/

Citation

From within R, enter citation("DNABarcodeCompatibility"):

Jacques Boutet de Monvel, Raphaël Etournay. DNABarcodeCompatibility: A Tool for Optimizing Combinations of DNA Barcodes Used in Multiplexed Experiments on Next Generation Sequencing Platforms. doi:10.18129/B9.bioc.DNABarcodeCompatibility, R package version 1.28.0, https://bioconductor.org/packages/DNABarcodeCompatibility.

Generated from the package metadata; it may differ from the package's own citation.

Documentation

Download

Follow the installation instructions to use this package in your R session.

Source packageDNABarcodeCompatibility_1.28.0.tar.gz
Windows binary (x86_64)DNABarcodeCompatibility_1.28.0.zip
macOS binary (arm64)DNABarcodeCompatibility_1.28.0.tgz
macOS binary (x86_64)DNABarcodeCompatibility_1.28.0.tgz
Dependencies

Depends: R (>= 3.6.0)

Imports: dplyr, tidyr, numbers, purrr, stringr, stats, utils, methods, Rcpp (>= 0.11.2), BH

LinkingTo: Rcpp, BH

Suggests: knitr, rmarkdown, BiocStyle, testthat