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Differential Enrichment analysis of Proteomics data

Bioconductor version: Release (3.19)

This package provides an integrated analysis workflow for robust and reproducible analysis of mass spectrometry proteomics data for differential protein expression or differential enrichment. It requires tabular input (e.g. txt files) as generated by quantitative analysis softwares of raw mass spectrometry data, such as MaxQuant or IsobarQuant. Functions are provided for data preparation, filtering, variance normalization and imputation of missing values, as well as statistical testing of differentially enriched / expressed proteins. It also includes tools to check intermediate steps in the workflow, such as normalization and missing values imputation. Finally, visualization tools are provided to explore the results, including heatmap, volcano plot and barplot representations. For scientists with limited experience in R, the package also contains wrapper functions that entail the complete analysis workflow and generate a report. Even easier to use are the interactive Shiny apps that are provided by the package.

Author: Arne Smits [cre, aut], Wolfgang Huber [aut]

Maintainer: Arne Smits <smits.arne at>

Citation (from within R, enter citation("DEP")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

DEP: Introduction HTML R Script
DEP: Missing value handling HTML R Script
Reference Manual PDF


biocViews DataRepresentation, DifferentialExpression, ImmunoOncology, MassSpectrometry, Proteomics, Software
Version 1.26.0
In Bioconductor since BioC 3.6 (R-3.4) (6.5 years)
License Artistic-2.0
Depends R (>= 3.5)
Imports ggplot2, dplyr, purrr, readr, tibble, tidyr, SummarizedExperiment(>= 1.11.5), MSnbase, limma, vsn, fdrtool, ggrepel, ComplexHeatmap, RColorBrewer, circlize, shiny, shinydashboard, DT, rmarkdown, assertthat, gridExtra, grid, stats, imputeLCMD, cluster
System Requirements
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Suggests testthat, enrichR, knitr, BiocStyle
Linking To
Depends On Me
Imports Me
Suggests Me proDA, RforProteomics
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Follow Installation instructions to use this package in your R session.

Source Package DEP_1.26.0.tar.gz
Windows Binary (64-bit only)
macOS Binary (x86_64) DEP_1.26.0.tgz
macOS Binary (arm64) DEP_1.26.0.tgz
Source Repository git clone
Source Repository (Developer Access) git clone
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