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posDemux

This is the released version of posDemux; for the devel version, see posDemux.

All versions 3.24 (devel), 3.23 (release)

Positional combinatorial sequence demultiplexer


Bioconductor version: Release (3.23)

Demultiplexing and filtering utilities intended for reads with combinatorial barcodes (i.e. PETRI-seq and SPLiT-seq). The demultiplexer algorithm uses the position of the segments to extract and compare the barcodes with the reference (whitelist). A Shiny application is provided to interactively select cutoffs for which barcode combinations to keep.

Author: Jakob Peder Pettersen [aut, cre] ORCID iD ORCID: 0000-0002-3485-1634 , Centre for new antibacterial strategies (CANS) [fnd]

Maintainer: Jakob Peder Pettersen <jakobpeder.pettersen at gmail.com>

Citation (from within R, enter citation("posDemux")):

Jakob Peder Pettersen. posDemux: Positional combinatorial sequence demultiplexer. doi:10.18129/B9.bioc.posDemux, R package version 1.0.0, https://bioconductor.org/packages/posDemux.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("posDemux")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("posDemux")
Introduction to combinatorial demultiplexing HTML R Script
Demultiplexing with streaming HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews RNASeq, SequenceMatching, Sequencing, Software
Version1.0.0
In Bioconductor sinceBioC 3.23 (R-4.6) (< 6 months)
License AGPL (>= 3)
Depends R (>= 4.5.0)
Imports Biostrings, ggplot2, methods, assertthat, glue, magrittr, dplyr, rlang, ShortRead, readr, shiny, purrr
System Requirements
URLhttps://github.com/yaccos/posDemux https://yaccos.github.io/posDemux/
Bug Reportshttps://github.com/yaccos/posDemux/issues
See More
Suggests testthat, devtools, DNABarcodes, knitr, rmarkdown, tibble, tidyr, BiocStyle, RefManageR, sessioninfo, DBI, chunked, RSQLite, dbplyr
Linking To Rcpp, Biostrings, IRanges, S4Vectors, XVector
Enhances
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package posDemux_1.0.0.tar.gz
Windows Binary (x86_64) posDemux_1.0.0.zip (64-bit only)
macOS Binary (big-sur-x86_64) posDemux_1.0.0.tgz
macOS Binary (sonoma-arm64) posDemux_1.0.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/posDemux
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/posDemux
Package Short Url https://bioconductor.org/packages/posDemux/
Package Downloads ReportDownload Stats