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SCArray.sat

This is the development version of SCArray.sat; for the stable release version, see SCArray.sat.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17

Large-scale single-cell RNA-seq data analysis using GDS files and Seurat


Bioconductor version: Development (3.24)

Extends the Seurat classes and functions to support Genomic Data Structure (GDS) files as a DelayedArray backend for data representation. It relies on the implementation of GDS-based DelayedMatrix in the SCArray package to represent single cell RNA-seq data. The common optimized algorithms leveraging GDS-based and single cell-specific DelayedMatrix (SC_GDSMatrix) are implemented in the SCArray package. SCArray.sat introduces a new SCArrayAssay class (derived from the Seurat Assay), which wraps raw counts, normalized expressions and scaled data matrix based on GDS-specific DelayedMatrix. It is designed to integrate seamlessly with the Seurat package to provide common data analysis in the SeuratObject-based workflow. Compared with Seurat, SCArray.sat significantly reduces the memory usage without downsampling and can be applied to very large datasets.

Author: Xiuwen Zheng [aut, cre] ORCID iD ORCID: 0000-0002-1390-0708 , Seurat contributors [ctb] (for the classes and methods defined in Seurat)

Maintainer: Xiuwen Zheng <xiuwen.zheng at abbvie.com>

Citation (from within R, enter citation("SCArray.sat")):

Xiuwen Zheng. SCArray.sat: Large-scale single-cell RNA-seq data analysis using GDS files and Seurat. doi:10.18129/B9.bioc.SCArray.sat, R package version 1.13.0, https://bioconductor.org/packages/SCArray.sat.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("SCArray.sat")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("SCArray.sat")
scRNA-seq data analysis with GDS files and Seurat HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews DataImport, DataRepresentation, RNASeq, SingleCell, Software
Version1.13.0
In Bioconductor sinceBioC 3.17 (R-4.3) (3.5 years)
License GPL-3
Depends methods, SCArray (>= 1.13.1), SeuratObject (>= 5.0), Seurat (>= 5.0)
Imports S4Vectors, utils, stats, BiocGenerics, BiocParallel, gdsfmt, DelayedArray, BiocSingular, SummarizedExperiment, Matrix
System Requirements
URL
Bug Reportshttps://github.com/AbbVie-ComputationalGenomics/SCArray/issues
See More
Suggests future, RUnit, knitr, markdown, rmarkdown, BiocStyle
Linking To
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Depends On Me
Imports Me
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Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package SCArray.sat_1.13.0.tar.gz
Windows Binary (x86_64) SCArray.sat_1.13.0.zip
macOS Binary (big-sur-x86_64) SCArray.sat_1.13.0.tgz
macOS Binary (sonoma-arm64) SCArray.sat_1.13.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/SCArray.sat
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/SCArray.sat
Package Short Url https://bioconductor.org/packages/SCArray.sat/
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