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crisprBowtie

This is the released version of crisprBowtie; for the devel version, see crisprBowtie.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17, 3.16, 3.15

Bowtie-based alignment of CRISPR gRNA spacer sequences


Bioconductor version: Release (3.23)

Provides a user-friendly interface to map on-targets and off-targets of CRISPR gRNA spacer sequences using bowtie. The alignment is fast, and can be performed using either commonly-used or custom CRISPR nucleases. The alignment can work with any reference or custom genomes. Both DNA- and RNA-targeting nucleases are supported.

Author: Jean-Philippe Fortin [aut, cre]

Maintainer: Jean-Philippe Fortin <fortin946 at gmail.com>

Citation (from within R, enter citation("crisprBowtie")):

Jean-Philippe Fortin. crisprBowtie: Bowtie-based alignment of CRISPR gRNA spacer sequences. doi:10.18129/B9.bioc.crisprBowtie, R package version 1.16.0, https://bioconductor.org/packages/crisprBowtie.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("crisprBowtie")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("crisprBowtie")
Introduction to crisprBowtie HTML R Script
Reference ManualPDF
NEWSText
LICENSEText

Details

biocViews Alignment, CRISPR, FunctionalGenomics, Software
Version1.16.0
In Bioconductor sinceBioC 3.15 (R-4.2) (4.5 years)
License MIT + file LICENSE
Depends methods
Imports BiocGenerics, Biostrings, BSgenome, crisprBase (>= 0.99.15), Seqinfo, GenomicRanges, IRanges, Rbowtie, readr, stats, stringr, utils
System Requirements
URLhttps://github.com/crisprVerse/crisprBowtie
Bug Reportshttps://github.com/crisprVerse/crisprBowtie/issues
See More
Suggests BiocStyle, BSgenome.Hsapiens.UCSC.hg38, knitr, rmarkdown, testthat
Linking To
Enhances
Depends On Me
Imports Me crisprDesign, crisprVerse
Suggests Me
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package crisprBowtie_1.16.0.tar.gz
Windows Binary (x86_64) crisprBowtie_1.16.0.zip
macOS Binary (big-sur-x86_64) crisprBowtie_1.16.0.tgz
macOS Binary (sonoma-arm64) crisprBowtie_1.16.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/crisprBowtie
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/crisprBowtie
Package Short Url https://bioconductor.org/packages/crisprBowtie/
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