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CySA

This is the development version of CySA; to use it, please install the devel version of Bioconductor.

Interactive Cluster Selector for Cytometry Data


Bioconductor version: Development (3.24)

CySA provides an interactive Shiny-based tool for analyzing flow and mass cytometry data. It supports self-organizing map (SOM) based clustering, dimension reduction (t-SNE, UMAP, PCA), and statistical comparison of cell populations across sample groups. Users can select cell clusters interactively via 2D scatter plots, dendrograms, or SOM node grids, and explore marker expression patterns across the selected populations.

Author: Bernd Jagla [aut, cre] ORCID iD ORCID: 0000-0002-7696-0484

Maintainer: Bernd Jagla <bernd.jagla at pasteur.fr>

Citation (from within R, enter citation("CySA")):
Seminal Bioconductor project articles:

Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M (2015). "Orchestrating high-throughput genomic analysis with Bioconductor." Nature Methods, 12(2), 115–121. doi:10.1038/nmeth.3252.

Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JYH, Zhang J (2004). "Bioconductor: open software development for computational biology and bioinformatics." Genome Biology, 5(10), R80. doi:10.1186/gb-2004-5-10-r80.

Installation

To install this package, start R (version "4.6") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("CySA")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

Reference Manual PDF

Details

biocViews Clustering, FlowCytometry, ShinyApps, SingleCell, Software, Visualization
Version 0.99.28
In Bioconductor since BioC 3.24 (R-4.6)
License MIT + file LICENSE
Depends R (>= 4.5.0)
Imports shiny, shinydashboard, shinydashboardPlus, shinyjs, shinyjqui, htmltools, SingleCellExperiment, S4Vectors, SummarizedExperiment, Matrix, tidyselect, matrixStats, RColorBrewer, CATALYST, FlowSOM, dplyr, tidyr, tibble, data.table, stringr, purrr, reshape2, rlang, raster, ggplot2, plotly, viridis, dendextend, ComplexHeatmap, cowplot, Rtsne, umap, DT, collapsibleTree, withr
System Requirements
URL https://github.com/baj12/CySA
Bug Reports https://github.com/baj12/CySA/issues
See More
Suggests KernSmooth, knitr, jsonlite, rmarkdown, shinytest2, testthat (>= 3.0.0)
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package
Windows Binary (x86_64)
macOS Binary (big-sur-x86_64)
macOS Binary (sonoma-arm64)
Source Repository git clone https://git.bioconductor.org/packages/CySA
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/CySA
Package Short Url https://bioconductor.org/packages/CySA/
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