DOI: 10.18129/B9.bioc.twoddpcr    

Classify 2-d Droplet Digital PCR (ddPCR) data and quantify the number of starting molecules

Bioconductor version: Release (3.6)

The twoddpcr package takes Droplet Digital PCR (ddPCR) droplet amplitude data from Bio-Rad's QuantaSoft and can classify the droplets. A summary of the positive/negative droplet counts can be generated, which can then be used to estimate the number of molecules using the Poisson distribution. This is the first open source package that facilitates the automatic classification of general two channel ddPCR data. Previous work includes 'definetherain' (Jones et al., 2014) and 'ddpcRquant' (Trypsteen et al., 2015) which both handle one channel ddPCR experiments only. The 'ddpcr' package available on CRAN (Attali et al., 2016) supports automatic gating of a specific class of two channel ddPCR experiments only.

Author: Anthony Chiu [aut, cre]

Maintainer: Anthony Chiu <anthony.chiu at>

Citation (from within R, enter citation("twoddpcr")):


To install this package, start R and enter:

## try http:// if https:// URLs are not supported


HTML R Script twoddpcr: A package for Droplet Digital PCR analysis
PDF   Reference Manual
Text   NEWS


biocViews Classification, Software, ddPCR
Version 1.2.1
In Bioconductor since BioC 3.5 (R-3.4) (1 year)
License GPL-3
Depends R (>= 3.4)
Imports class, ggplot2, hexbin, methods, scales, shiny, stats, utils, RColorBrewer, S4Vectors
Suggests devtools, knitr, reshape2, rmarkdown, testthat, BiocStyle
Depends On Me
Imports Me
Suggests Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package twoddpcr_1.2.1.tar.gz
Windows Binary
Mac OS X 10.11 (El Capitan) twoddpcr_1.2.1.tgz
Source Repository git clone
Package Short Url
Package Downloads Report Download Stats
Old Source Packages for BioC 3.6 Source Archive

Documentation »


R / CRAN packages and documentation

Support »

Please read the posting guide. Post questions about Bioconductor to one of the following locations: