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crisprBowtie

Bowtie-based alignment of CRISPR gRNA spacer sequences

Bioconductor version: 3.23 · Package version: 1.16.0

Other Bioconductor versions

devel is the development version; release is the current stable one.

3.24 (devel), 3.23 (release)

Provides a user-friendly interface to map on-targets and off-targets of CRISPR gRNA spacer sequences using bowtie. The alignment is fast, and can be performed using either commonly-used or custom CRISPR nucleases. The alignment can work with any reference or custom genomes. Both DNA- and RNA-targeting nucleases are supported.

DOI: 10.18129/B9.bioc.crisprBowtie

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("crisprBowtie")

Details

MaintainerJean-Philippe Fortin <fortin946@gmail.com>
AuthorJean-Philippe Fortin [aut, cre]
LicenseMIT + file LICENSE
URLhttps://github.com/crisprVerse/crisprBowtie
Bug Reportshttps://github.com/crisprVerse/crisprBowtie/issues
Source branchRELEASE_3_23
Build report Bioconductor build system, r-universe
biocViewsAlignment, CRISPR, FunctionalGenomics, Software
Package Short Url https://bioconductor.org/packages/crisprBowtie/

Citation

From within R, enter citation("crisprBowtie"):

Jean-Philippe Fortin. crisprBowtie: Bowtie-based alignment of CRISPR gRNA spacer sequences. doi:10.18129/B9.bioc.crisprBowtie, R package version 1.16.0, https://bioconductor.org/packages/crisprBowtie.

Generated from the package metadata; it may differ from the package's own citation.

Documentation

Download

Follow the installation instructions to use this package in your R session.

Source packagecrisprBowtie_1.16.0.tar.gz
Windows binary (x86_64)crisprBowtie_1.16.0.zip
macOS binary (arm64)crisprBowtie_1.16.0.tgz
macOS binary (x86_64)crisprBowtie_1.16.0.tgz
Dependencies

Depends: methods

Imports: BiocGenerics, Biostrings, BSgenome, crisprBase (>= 0.99.15), Seqinfo, GenomicRanges, IRanges, Rbowtie, readr, stats, stringr, utils

Suggests: BiocStyle, BSgenome.Hsapiens.UCSC.hg38, knitr, rmarkdown, testthat

Reverse dependencies

Imports Me (2): crisprDesign, crisprVerse