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UMI4Cats

This is the released version of UMI4Cats; for the devel version, see UMI4Cats.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17, 3.16, 3.15, 3.14, 3.13, 3.12

UMI4Cats: Processing, analysis and visualization of UMI-4C chromatin contact data


Bioconductor version: Release (3.23)

UMI-4C is a technique that allows characterization of 3D chromatin interactions with a bait of interest, taking advantage of a sonication step to produce unique molecular identifiers (UMIs) that help remove duplication bias, thus allowing a better differential comparsion of chromatin interactions between conditions. This package allows processing of UMI-4C data, starting from FastQ files provided by the sequencing facility. It provides two statistical methods for detecting differential contacts and includes a visualization function to plot integrated information from a UMI-4C assay.

Author: Mireia Ramos-Rodriguez [aut, cre] ORCID iD ORCID: 0000-0001-8083-2445 , Marc Subirana-Granes [aut], Lorenzo Pasquali [aut]

Maintainer: Mireia Ramos-Rodriguez <mireiarr9 at gmail.com>

Citation (from within R, enter citation("UMI4Cats")):

Mireia Ramos-Rodriguez, Marc Subirana-Granes, Lorenzo Pasquali. UMI4Cats: UMI4Cats: Processing, analysis and visualization of UMI-4C chromatin contact data. doi:10.18129/B9.bioc.UMI4Cats, R package version 1.22.0, https://bioconductor.org/packages/UMI4Cats.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("UMI4Cats")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("UMI4Cats")
Analyzing UMI-4C data with UMI4Cats HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews Alignment, Coverage, Normalization, Preprocessing, QualityControl, Sequencing, Software, Visualization
Version1.22.0
In Bioconductor sinceBioC 3.12 (R-4.0) (6 years)
License Artistic-2.0
Depends R (>= 4.1.0), SummarizedExperiment
Imports magick, cowplot, scales, GenomicRanges, ShortRead, zoo, ggplot2, reshape2, regioneR, IRanges, S4Vectors, dplyr, BSgenome, Biostrings, DESeq2, R.utils, Rsamtools, stringr, Rbowtie2, methods, GenomeInfoDb, GenomicAlignments, RColorBrewer, utils, grDevices, stats, annotate, rlang, GenomicFeatures, BiocFileCache, rappdirs, fda, BiocGenerics
System Requirements
URLhttps://github.com/Pasquali-lab/UMI4Cats
Bug Reportshttps://github.com/Pasquali-lab/UMI4Cats/issues
See More
Suggests knitr, rmarkdown, BiocStyle, org.Hs.eg.db, TxDb.Hsapiens.UCSC.hg19.knownGene, BSgenome.Hsapiens.UCSC.hg19, tidyr, testthat
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package UMI4Cats_1.22.0.tar.gz
Windows Binary (x86_64) UMI4Cats_1.22.0.zip
macOS Binary (big-sur-x86_64) UMI4Cats_1.22.0.tgz
macOS Binary (sonoma-arm64) UMI4Cats_1.22.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/UMI4Cats
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/UMI4Cats
Package Short Url https://bioconductor.org/packages/UMI4Cats/
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