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A package for importing and analyzing data from Roche's Genome Sequencer System

Bioconductor version: Release (3.19)

The R453Plus1 Toolbox comprises useful functions for the analysis of data generated by Roche's 454 sequencing platform. It adds functions for quality assurance as well as for annotation and visualization of detected variants, complementing the software tools shipped by Roche with their product. Further, a pipeline for the detection of structural variants is provided.

Author: Hans-Ulrich Klein, Christoph Bartenhagen, Christian Ruckert

Maintainer: Hans-Ulrich Klein <h.klein at>

Citation (from within R, enter citation("R453Plus1Toolbox")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

A package for importing and analyzing data from Roche's Genome Sequencer System PDF R Script
Reference Manual PDF


biocViews DataImport, DataRepresentation, Infrastructure, QualityControl, ReportWriting, Sequencing, Software, Visualization
Version 1.54.0
In Bioconductor since BioC 2.7 (R-2.12) (13.5 years)
License LGPL-3
Depends R (>= 2.12.0), methods, VariantAnnotation(>= 1.25.11), Biostrings(>= 2.47.6), pwalign, Biobase
Imports utils, grDevices, graphics, stats, tools, xtable, R2HTML, TeachingDemos, BiocGenerics, S4Vectors(>= 0.17.25), IRanges(>= 2.13.12), XVector, GenomicRanges(>= 1.31.8), SummarizedExperiment, biomaRt, BSgenome(>= 1.47.3), Rsamtools, ShortRead(>= 1.37.1)
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Suggests rtracklayer, BSgenome.Hsapiens.UCSC.hg19, BSgenome.Scerevisiae.UCSC.sacCer2
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Source Package R453Plus1Toolbox_1.54.0.tar.gz
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