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OSCA.advanced

This is the released version of OSCA.advanced; for the devel version, see OSCA.advanced.

All versions 3.24 (devel), 3.23 (release)

Advanced Single-Cell Analysis


Bioconductor version: Release (3.23)

Deploys the advanced analysis chapters for the "Orchestrating Single Cell Analysis with Bioconductor" book. This describes the more complex steps of a single-cell RNA-seq analysis ranging from doublet detection, cell cycle assignment, specific steps for processing droplet data, nuclei-specific analyses, trajectory analyses, integrated analyses with protein abundances, and interactive visualization. It also elaborates on some of the basic analysis steps, focusing on alternative strategies and theoretical considerations. It is intended for readers who are already familiar with basic single-cell analyses, possibly after reading some of the prior books in this collection.

Author: Robert Amezquita [aut], Aaron Lun [aut], Stephanie Hicks [aut], Raphael Gottardo [aut], Ludwig Geistlinger [cre], Peter Hickey [ctb]

Maintainer: Ludwig Geistlinger <ludwig_geistlinger at hms.harvard.edu>

Citation (from within R, enter citation("OSCA.advanced")):

Robert Amezquita, Aaron Lun, Stephanie Hicks, Raphael Gottardo. OSCA.advanced: Advanced Single-Cell Analysis. doi:10.18129/B9.bioc.OSCA.advanced, R package version 1.20.1, https://bioconductor.org/packages/OSCA.advanced.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("OSCA.advanced")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

No vignettes available
Reference ManualPDF

Details

biocViews ImmunoOncologyWorkflow, SingleCellWorkflow, Software, Workflow
Version1.20.1
In Bioconductor sinceBioC 3.23 (R-4.6) (< 6 months)
License CC BY 4.0
Depends AnnotationHub, batchelor, BiocFileCache, BiocNeighbors, BiocParallel, BiocSingular, bluster, clustree, corral, densvis, DropletTestFiles, DropletUtils, edgeR, ggbeeswarm, ggplot2, gridExtra, igraph, iSEE, limma, msigdbr, mumosa, org.Mm.eg.db, pheatmap, rebook, rmarkdown, Rtsne, scater, scDblFinder, scPCA, scran, scrapper, scRNAseq (>= 2.17.9), scuttle, SingleR, slingshot, snifter, TENxBrainData, tradeSeq, TSCAN, velociraptor, viridis
Imports OSCA.workflows, OSCA.basic, OSCA.multisample
System Requirements
URLhttps://github.com/OSCA-source/OSCA.advanced
See More
Suggests rmarkdown, knitr
Linking To
Enhances
Depends On Me
Imports Me
Suggests Me OSCA, OSCA.basic
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package
Windows Binary (x86_64)
macOS Binary (big-sur-x86_64)
macOS Binary (sonoma-arm64)
Source Repositorygit clone https://git.bioconductor.org/packages/OSCA.advanced
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/OSCA.advanced
Package Short Url https://bioconductor.org/packages/OSCA.advanced/
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