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fishash

This is the development version of fishash; to use it, please install the devel version of Bioconductor.

Cell Hashing with One-Sided Fisher Test


Bioconductor version: Development (3.24)

Assigns guide RNAs or other genetic perturbations to cells in single-cell sequencing experiments using a one-sided Fisher's exact test. Implements an iterative refitting procedure to mitigate Simpson's paradox, supports multiple false discovery rate correction methods (Benjamini-Hochberg, Benjamini-Yekutueli, and Guo & Sarkar 2020), and provides simulation utilities for benchmarking demultiplexing methods. Results are returned as SummarizedExperiment objects.

Author: Jack Kamm [aut, cre]

Maintainer: Jack Kamm <jackkamm at gmail.com>

Citation (from within R, enter citation("fishash")):

Jack Kamm. fishash: Cell Hashing with One-Sided Fisher Test. doi:10.18129/B9.bioc.fishash, R package version 0.99.5, https://bioconductor.org/packages/fishash.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("fishash")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("fishash")
Using fishash to assign gRNAs in Perturbseq data HTML R Script
Reference ManualPDF
NEWSText
LICENSEText

Details

biocViews Preprocessing, Sequencing, SingleCell, Software, StatisticalMethod
Version0.99.5
In Bioconductor sinceBioC 3.24 (R-4.6)
License MIT + file LICENSE
Depends R (>= 4.5.0)
Imports dplyr, extraDistr, ggplot2, Matrix, methods, nnet, patchwork, rlang, S4Vectors, SingleCellExperiment, SummarizedExperiment, sparseMatrixStats
System Requirements
URLhttps://github.com/jackkamm/fishash
Bug Reportshttps://github.com/jackkamm/fishash/issues
See More
Suggests BiocStyle, ComplexHeatmap, ggExtra, glmGamPoi, knitr, rmarkdown, testthat (>= 3.0.0)
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Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package fishash_0.99.5.tar.gz
Windows Binary (x86_64) fishash_0.99.5.zip
macOS Binary (big-sur-x86_64) fishash_0.99.5.tgz
macOS Binary (sonoma-arm64) fishash_0.99.5.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/fishash
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/fishash
Package Short Url https://bioconductor.org/packages/fishash/
Package Downloads ReportDownload Stats