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HumanRetinaLRSData

This is the development version of HumanRetinaLRSData; for the stable release version, see HumanRetinaLRSData.

All versions 3.24 (devel), 3.23 (release)

Long-read RNA-seq gene count data from human retinal organoids


Bioconductor version: Development (3.24)

Dataset package containing gene and isoform count matrices, and sample metadata for long-read direct cDNA sequencing of human retinal organoids, 2D retinal ganglion cell (RGC) cultures, and flowthrough fractions from H9 and EP1 iPSC cell lines. Data were generated using Oxford Nanopore Technology (ONT) direct cDNA sequencing and mapped to the GRCh38 reference genome (GENCODE v46 annotation). The package provides accessor functions returning SummarizedExperiment objects for gene-level counts, isoform-level counts, and a matrix of allele-specific expression (ASE) gene counts. Data files are stored in flat CSV format in an Open Science Framework (OSF) repository and cached locally via BiocFileCache.

Author: Sowmya Parthiban [aut, cre]

Maintainer: Sowmya Parthiban <sparthi1 at jhu.edu>

Citation (from within R, enter citation("HumanRetinaLRSData")):

Sowmya Parthiban. HumanRetinaLRSData: Long-read RNA-seq gene count data from human retinal organoids. doi:10.18129/B9.bioc.HumanRetinaLRSData, R package version 1.1.0, https://bioconductor.org/packages/HumanRetinaLRSData.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("HumanRetinaLRSData")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("HumanRetinaLRSData")
Retinal Organoids counts data: Gene and Isoform Level Exploration HTML R Script
Reference ManualPDF
LICENSEText

Details

biocViews CellCulture, ExperimentData, ExpressionData, Homo_sapiens_Data, RNASeqData
Version1.1.0
In Bioconductor sinceBioC 3.23 (R-4.6) (< 6 months)
License MIT + file LICENSE
Depends R (>= 4.6.0), SummarizedExperiment
Imports osfr, BiocFileCache
System Requirements
URLhttps://github.com/sparthib/HumanRetinaLrsData
Bug Reportshttps://github.com/sparthib/HumanRetinaLrsData/issues
See More
Suggests testthat, knitr, rmarkdown, ggplot2, ggrepel
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package HumanRetinaLRSData_1.1.0.tar.gz
Windows Binary (x86_64)
macOS Binary (big-sur-x86_64)
macOS Binary (sonoma-arm64)
Source Repositorygit clone https://git.bioconductor.org/packages/HumanRetinaLRSData
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/HumanRetinaLRSData
Package Short Url https://bioconductor.org/packages/HumanRetinaLRSData/
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