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staRgate

This is the development version of staRgate; for the stable release version, see staRgate.

All versions 3.24 (devel), 3.23 (release)

Automated gating pipeline for flow cytometry analysis to characterize the lineage, differentiation, and functional states of T-cells


Bioconductor version: Development (3.24)

An R-based automated gating pipeline for flow cytometry data designed to mimic the manual gating strategy of defining flow biomarker positive populations relative to a unimodal background population to include cells with varying intensities of marker expression. The pipeline’s main feature is a flexible density-based gating strategy capable of capturing varying scenarios based on marker expression patterns to analyze a 29-marker flow panel that characterizes T-cell lineage, differentiation, and functional states.

Author: Jasme Lee [aut, cre] ORCID iD ORCID: 0009-0006-4492-4872 , Matthew Adamow [aut], Colleen Maher [aut], Xiyu Peng [aut], Phillip Wong [aut], Fiona Ehrich [aut], Michael A Postow [aut], Margaret K Callahan [aut], Ronglai Shen [aut], Katherine S Panageas [aut], V foundation [fnd], MSK-MIND [fnd], NIH R01CA276286 [fnd], NIH P30CA008748 [fnd]

Maintainer: Jasme Lee <leej22 at mskcc.org>

Citation (from within R, enter citation("staRgate")):

Jasme Lee, Matthew Adamow, Colleen Maher, Xiyu Peng, Phillip Wong, Fiona Ehrich, Michael A Postow, Margaret K Callahan, Ronglai Shen, Katherine S Panageas. staRgate: Automated gating pipeline for flow cytometry analysis to characterize the lineage, differentiation, and functional states of T-cells. doi:10.18129/B9.bioc.staRgate, R package version 1.1.0, https://bioconductor.org/packages/staRgate.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("staRgate")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("staRgate")
Tutorial: Running the pipeline HTML R Script
Reference ManualPDF
LICENSEText

Details

biocViews FlowCytometry, ImmunoOncology, Preprocessing, Software
Version1.1.0
In Bioconductor sinceBioC 3.23 (R-4.6) (< 6 months)
License MIT + file LICENSE
Depends R (>= 4.3.0)
Imports dplyr, janitor, purrr, rlang, stringr, tidyr, flowCore, flowWorkspace, glue, tibble
System Requirements
URLhttps://bioconductor.org/packages/staRgate https://leejasme.github.io/staRgate
Bug Reportshttps://github.com/leejasme/staRgate/issues
See More
Suggests flowAI, ggplot2, gt, knitr, openCyto, ggcyto, rmarkdown, data.table, here, testthat (>= 3.0.0), BiocStyle
Linking To
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Depends On Me
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Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package staRgate_1.1.0.tar.gz
Windows Binary (x86_64) staRgate_1.1.0.zip
macOS Binary (big-sur-x86_64) staRgate_1.1.0.tgz
macOS Binary (sonoma-arm64) staRgate_1.1.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/staRgate
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/staRgate
Package Short Url https://bioconductor.org/packages/staRgate/
Package Downloads ReportDownload Stats