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scPipe

This is the development version of scPipe; for the stable release version, see scPipe.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17, 3.16, 3.15, 3.14, 3.13, 3.12, 3.11, 3.10, 3.9, 3.8, 3.7, 3.6

Pipeline for single cell multi-omic data pre-processing


Bioconductor version: Development (3.24)

A preprocessing pipeline for single cell RNA-seq/ATAC-seq data that starts from the fastq files and produces a feature count matrix with associated quality control information. It can process fastq data generated by CEL-seq, MARS-seq, Drop-seq, Chromium 10x and SMART-seq protocols.

Author: Luyi Tian [aut], Shian Su [aut, cre], Shalin Naik [ctb], Shani Amarasinghe [aut], Oliver Voogd [aut], Phil Yang [aut], Matthew Ritchie [ctb]

Maintainer: Shian Su <su.s at wehi.edu.au>

Citation (from within R, enter citation("scPipe")):

Luyi Tian, Shian Su, Shani Amarasinghe, Oliver Voogd, Phil Yang. scPipe: Pipeline for single cell multi-omic data pre-processing. doi:10.18129/B9.bioc.scPipe, R package version 2.13.1, https://bioconductor.org/packages/scPipe.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("scPipe")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("scPipe")
scPipe: a flexible data preprocessing pipeline for scATAC-seq data HTML R Script
scPipe: a flexible data preprocessing pipeline for 3' end scRNA-seq data HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews DataImport, GeneExpression, GenomeAnnotation, ImmunoOncology, Preprocessing, QualityControl, RNASeq, SequenceMatching, Sequencing, SingleCell, Software, Visualization
Version2.13.1
In Bioconductor sinceBioC 3.6 (R-3.4) (9 years)
License GPL (>= 2)
Depends R (>= 4.2.0), SingleCellExperiment
Imports AnnotationDbi, basilisk, BiocGenerics, biomaRt, Biostrings, data.table, dplyr, DropletUtils, flexmix, GenomicRanges, GenomicAlignments, GGally, ggplot2, glue (>= 1.3.0), grDevices, graphics, hash, IRanges, magrittr, MASS, Matrix (>= 1.5.0), mclust, methods, MultiAssayExperiment, org.Hs.eg.db, org.Mm.eg.db, purrr, Rcpp (>= 0.11.3), reshape, reticulate, Rhtslib, rlang, robustbase, Rsamtools, Rsubread, rtracklayer, SummarizedExperiment, S4Vectors, scales, stats, stringr, tibble, tidyr, tools, utils, vctrs (>= 0.5.2)
System RequirementsC++11, GNU make
URLhttps://github.com/LuyiTian/scPipe
Bug Reportshttps://github.com/LuyiTian/scPipe
See More
Suggests BiocStyle, DT, GenomicFeatures, grid, igraph, kableExtra, knitr, locStra, plotly, rmarkdown, RColorBrewer, readr, reshape2, RANN, shiny, scater (>= 1.11.0), testthat, xml2, umap
Linking To Rcpp, Rhtslib (>= 1.13.1), testthat
Enhances
Depends On Me
Imports Me stPipe
Suggests Me
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package scPipe_2.13.1.tar.gz
Windows Binary (x86_64) scPipe_2.13.1.zip (64-bit only)
macOS Binary (big-sur-x86_64) scPipe_2.13.1.tgz
macOS Binary (sonoma-arm64) scPipe_2.13.1.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/scPipe
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/scPipe
Package Short Url https://bioconductor.org/packages/scPipe/
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