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openPrimeR

This is the development version of openPrimeR; for the stable release version, see openPrimeR.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17, 3.16, 3.15, 3.14, 3.13, 3.12, 3.11, 3.10, 3.9, 3.8, 3.7, 3.6

Multiplex PCR Primer Design and Analysis


Bioconductor version: Development (3.24)

An implementation of methods for designing, evaluating, and comparing primer sets for multiplex PCR. Primers are designed by solving a set cover problem such that the number of covered template sequences is maximized with the smallest possible set of primers. To guarantee that high-quality primers are generated, only primers fulfilling constraints on their physicochemical properties are selected. A Shiny app providing a user interface for the functionalities of this package is provided by the 'openPrimeRui' package.

Author: Matthias Döring [aut, cre], Nico Pfeifer [aut]

Maintainer: Matthias Döring <matthias-doering at gmx.de>

Citation (from within R, enter citation("openPrimeR")):

Matthias Döring, Nico Pfeifer. openPrimeR: Multiplex PCR Primer Design and Analysis. doi:10.18129/B9.bioc.openPrimeR, R package version 1.35.0, https://bioconductor.org/packages/openPrimeR.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("openPrimeR")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("openPrimeR")
openPrimeR HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews Coverage, MultipleComparison, Software, Technology
Version1.35.0
In Bioconductor sinceBioC 3.6 (R-3.4) (9 years)
License GPL-2
Depends R (>= 4.0.0)
Imports Biostrings (>= 2.38.4), pwalign, XML (>= 3.98-1.4), scales (>= 0.4.0), reshape2 (>= 1.4.1), seqinr (>= 3.3-3), IRanges (>= 2.4.8), GenomicRanges (>= 1.22.4), ggplot2 (>= 2.1.0), plyr (>= 1.8.4), dplyr (>= 0.5.0), stringdist (>= 0.9.4.1), stringr (>= 1.0.0), RColorBrewer (>= 1.1-2), DECIPHER (>= 1.16.1), lpSolveAPI (>= 5.5.2.0-17), digest (>= 0.6.9), Hmisc (>= 3.17-4), ape (>= 3.5), BiocGenerics (>= 0.16.1), S4Vectors (>= 0.8.11), foreach (>= 1.4.3), magrittr (>= 1.5), uniqtag (>= 1.0), openxlsx (>= 4.0.17), grid (>= 3.1.0), grDevices (>= 3.1.0), stats (>= 3.1.0), utils (>= 3.1.0), methods (>= 3.1.0)
System RequirementsMAFFT (>= 7.305), OligoArrayAux (>= 3.8), ViennaRNA (>= 2.4.1), MELTING (>= 5.1.1), Pandoc (>= 1.12.3)
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Suggests testthat (>= 1.0.2), knitr (>= 1.13), rmarkdown (>= 1.0), devtools (>= 1.12.0), doParallel (>= 1.0.10), pander (>= 0.6.0), learnr (>= 0.9)
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package openPrimeR_1.35.0.tar.gz
Windows Binary (x86_64) openPrimeR_1.35.0.zip
macOS Binary (big-sur-x86_64) openPrimeR_1.35.0.tgz
macOS Binary (sonoma-arm64) openPrimeR_1.35.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/openPrimeR
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/openPrimeR
Package Short Url https://bioconductor.org/packages/openPrimeR/
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