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CytoPipeline

This is the development version of CytoPipeline; for the stable release version, see CytoPipeline.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17

Automation and visualization of flow cytometry data analysis pipelines


Bioconductor version: Development (3.24)

This package provides support for automation and visualization of flow cytometry data analysis pipelines. In the current state, the package focuses on the preprocessing and quality control part. The framework is based on two main S4 classes, i.e. CytoPipeline and CytoProcessingStep. The pipeline steps are linked to corresponding R functions - that are either provided in the CytoPipeline package itself, or exported from a third party package, or coded by the user her/himself. The processing steps need to be specified centrally and explicitly using either a json input file or through step by step creation of a CytoPipeline object with dedicated methods. After having run the pipeline, obtained results at all steps can be retrieved and visualized thanks to file caching (the running facility uses a BiocFileCache implementation). The package provides also specific visualization tools like pipeline workflow summary display, and 1D/2D comparison plots of obtained flowFrames at various steps of the pipeline.

Author: Philippe Hauchamps [aut, cre] ORCID iD ORCID: 0000-0003-2865-1852 , Laurent Gatto [aut] ORCID iD ORCID: 0000-0002-1520-2268 , Dan Lin [ctb]

Maintainer: Philippe Hauchamps <philippe.hauchamps at uclouvain.be>

Citation (from within R, enter citation("CytoPipeline")):

Philippe Hauchamps, Laurent Gatto. CytoPipeline: Automation and visualization of flow cytometry data analysis pipelines. doi:10.18129/B9.bioc.CytoPipeline, R package version 1.13.1, https://bioconductor.org/packages/CytoPipeline.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("CytoPipeline")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("CytoPipeline")
Automation and Visualization of Flow Cytometry Data Analysis Pipelines HTML R Script
Demonstration of the CytoPipeline R package suite functionalities HTML R Script
Reference ManualPDF
NEWSText
LICENSEText

Details

biocViews FlowCytometry, ImmunoOncology, Preprocessing, QualityControl, Software, Visualization, WorkflowStep
Version1.13.1
In Bioconductor sinceBioC 3.17 (R-4.3) (3.5 years)
License GPL-3
Depends R (>= 4.4)
Imports methods, stats, utils, withr, rlang, ggplot2 (>= 3.4.1), ggcyto, BiocFileCache, BiocParallel, flowCore, PeacoQC, flowAI, diagram, jsonlite, scales
System Requirements
URLhttps://uclouvain-cbio.github.io/CytoPipeline
Bug Reportshttps://github.com/UCLouvain-CBIO/CytoPipeline/issues
See More
Suggests testthat (>= 3.0.0), vdiffr, diffviewer, knitr, rmarkdown, BiocStyle, reshape2, dplyr, CytoPipelineGUI
Linking To
Enhances
Depends On Me CytoPipelineGUI
Imports Me CytoMDS
Suggests Me
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package CytoPipeline_1.13.1.tar.gz
Windows Binary (x86_64) CytoPipeline_1.13.1.zip (64-bit only)
macOS Binary (big-sur-x86_64) CytoPipeline_1.13.1.tgz
macOS Binary (sonoma-arm64) CytoPipeline_1.13.1.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/CytoPipeline
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/CytoPipeline
Package Short Url https://bioconductor.org/packages/CytoPipeline/
Package Downloads ReportDownload Stats