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OAtools

This is the released version of OAtools; for the devel version, see OAtools.

Analysis of OpenArray PCR Data


Bioconductor version: Release (3.23)

Provides a suite of R functions to analyze gene expression experiments on the OpenArray real-time PCR platform. OAtools fits logistic regressions to fluorescence curves to distinguish between real amplification and false positives. OAtools supports data import, analysis, and visualization through plots and a dynamic HTML report.

Author: Aidan Shea [aut, cre] ORCID iD ORCID: 0009-0001-9256-7439

Maintainer: Aidan Shea <aidantshea at gmail.com>

Citation (from within R, enter citation("OAtools")):
Seminal Bioconductor project articles:

Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M (2015). "Orchestrating high-throughput genomic analysis with Bioconductor." Nature Methods, 12(2), 115–121. doi:10.1038/nmeth.3252.

Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JYH, Zhang J (2004). "Bioconductor: open software development for computational biology and bioinformatics." Genome Biology, 5(10), R80. doi:10.1186/gb-2004-5-10-r80.

Installation

To install this package, start R (version "4.6") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("OAtools")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("OAtools")
Analyzing OpenArray Gene Expression Data with OAtools HTML R Script
Reference Manual PDF

Details

biocViews DataImport, GeneExpression, Regression, Software, qPCR
Version 1.0.0
In Bioconductor since BioC 3.23 (R-4.6) (< 6 months)
License GPL (>= 3)
Depends R (>= 4.6)
Imports basilisk(>= 1.20.0), Biobase(>= 2.70.0), dplyr (>= 1.1.4), DT (>= 0.34.0), ggplot2 (>= 3.5.2), janitor (>= 2.2.1), methods (>= 4.5.2), plotly (>= 4.11.0), purrr (>= 1.2.0), ReadqPCR(>= 1.56.0), readxl (>= 1.4.5), reticulate (>= 1.43.0), rlang (>= 1.1.6), rmarkdown (>= 2.29), S4Vectors(>= 0.48.0), shiny (>= 1.13.0), SummarizedExperiment(>= 1.40.0), tibble (>= 3.3.0), tidyr (>= 1.3.1), writexl (>= 1.5.4)
System Requirements
URL https://github.com/uwvirology-ngs/OAtools
Bug Reports https://github.com/uwvirology-ngs/OAtools/issues
See More
Suggests testthat (>= 3.0.0), knitr, kableExtra (>= 1.4.0), NormqPCR(>= 1.56.0), BiocManager (>= 1.30.27)
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package OAtools_1.0.0.tar.gz
Windows Binary (x86_64) OAtools_1.0.0.zip
macOS Binary (big-sur-x86_64) OAtools_1.0.0.tgz
macOS Binary (sonoma-arm64) OAtools_1.0.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/OAtools
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/OAtools
Bioc Package Browser https://code.bioconductor.org/browse/OAtools/
Package Short Url https://bioconductor.org/packages/OAtools/
Package Downloads Report Download Stats