normalize450K
Preprocessing of Illumina Infinium 450K data
Bioconductor version: Release (3.20)
Precise measurements are important for epigenome-wide studies investigating DNA methylation in whole blood samples, where effect sizes are expected to be small in magnitude. The 450K platform is often affected by batch effects and proper preprocessing is recommended. This package provides functions to read and normalize 450K '.idat' files. The normalization corrects for dye bias and biases related to signal intensity and methylation of probes using local regression. No adjustment for probe type bias is performed to avoid the trade-off of precision for accuracy of beta-values.
Author: Jonathan Alexander Heiss
Maintainer: Jonathan Alexander Heiss <jonathan.heiss at posteo.de>
citation("normalize450K")):
Installation
To install this package, start R (version "4.4") and enter:
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("normalize450K")
For older versions of R, please refer to the appropriate Bioconductor release.
Documentation
To view documentation for the version of this package installed in your system, start R and enter:
browseVignettes("normalize450K")| Normalization of 450K data | R Script | |
| Reference Manual | ||
| LICENSE | Text |
Details
| biocViews | DNAMethylation, MethylationArray, Microarray, Normalization, Preprocessing, Software, TwoChannel |
| Version | 1.34.0 |
| In Bioconductor since | BioC 3.3 (R-3.3) (8.5 years) |
| License | BSD_2_clause + file LICENSE |
| Depends | R (>= 3.3), Biobase, illuminaio, quadprog |
| Imports | utils |
| System Requirements | |
| URL |
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| Build Report | Build Report |
Package Archives
Follow Installation instructions to use this package in your R session.
| Source Package | normalize450K_1.34.0.tar.gz |
| Windows Binary (x86_64) | normalize450K_1.34.0.zip |
| macOS Binary (x86_64) | normalize450K_1.34.0.tgz |
| macOS Binary (arm64) | normalize450K_1.33.0.tgz |
| Source Repository | git clone https://git.bioconductor.org/packages/normalize450K |
| Source Repository (Developer Access) | git clone git@git.bioconductor.org:packages/normalize450K |
| Bioc Package Browser | https://code.bioconductor.org/browse/normalize450K/ |
| Package Short Url | https://bioconductor.org/packages/normalize450K/ |
| Package Downloads Report | Download Stats |