DOI: 10.18129/B9.bioc.RnaSeqSampleSize    

This package is for version 3.14 of Bioconductor. This package has been removed from Bioconductor. For the last stable, up-to-date release version, see RnaSeqSampleSize.


Bioconductor version: Development (3.14)

RnaSeqSampleSize package provides a sample size calculation method based on negative binomial model and the exact test for assessing differential expression analysis of RNA-seq data. It controls FDR for multiple testing and utilizes the average read count and dispersion distributions from real data to estimate a more reliable sample size. It is also equipped with several unique features, including estimation for interested genes or pathway, power curve visualization, and parameter optimization.

Author: Shilin Zhao Developer [aut, cre], Chung-I Li Developer [aut], Yan Guo Developer [aut], Quanhu Sheng Developer [aut], Yu Shyr Developer [aut]

Maintainer: Shilin Zhao Developer <zhaoshilin at gmail.com>

Citation (from within R, enter citation("RnaSeqSampleSize")):


To install this package, start R (version "4.1") and enter:

if (!requireNamespace("BiocManager", quietly = TRUE))

# The following initializes usage of Bioc devel


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:



PDF R Script RnaSeqSampleSize: Sample size estimation by real data
PDF   Reference Manual
Text   NEWS


biocViews DifferentialExpression, ExperimentalDesign, GeneExpression, ImmunoOncology, RNASeq, Sequencing, Software
Version 2.3.0
In Bioconductor since BioC 3.1 (R-3.2) (6.5 years)
License GPL (>= 2)
Depends R (>= 4.0.0), RnaSeqSampleSizeData
Imports biomaRt, edgeR, heatmap3, matlab, KEGGREST, methods, grDevices, graphics, stats, utils, Rcpp (>= 0.11.2)
LinkingTo Rcpp
Suggests BiocStyle, knitr, testthat
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package RnaSeqSampleSize_2.3.0.tar.gz
Windows Binary RnaSeqSampleSize_2.3.0.zip
macOS 10.13 (High Sierra) RnaSeqSampleSize_2.3.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/RnaSeqSampleSize
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/RnaSeqSampleSize
Package Short Url https://bioconductor.org/packages/RnaSeqSampleSize/
Package Downloads Report Download Stats

Documentation »


R / CRAN packages and documentation

Support »

Please read the posting guide. Post questions about Bioconductor to one of the following locations: