Methylation Arrays

Martin Morgan (mtmorgan@fhcrc.org), Fred Hutchinson Cancer Research, Center, Seattle, WA, USA.
24 August 2014

E.g., HumanMethylation450k

  • Interrogate fragmented whole-genome DNA for methylationat specific genomic locations

Workflow

  1. Experimental design: replicate groups
  2. Wet-lab protocol
  3. beta: per-sample probe differential methylation
  4. Quality assessment
  5. Between-treatment differential methylation
  6. Interpretation

Illumina bead arrays