1 Introduction

This package produces AnVIL workspaces from R packages. An example uses the new Gen3 package as a basis for the Bioconductor-Package-Gen3 workspace (permission to access this workspace is required, but there are no restrictions on granting permission).

1.1 Package installation

If necessary, install the AnVILPublish library

if (!"AnVILPublish" %in% rownames(installed.packages()))

There are only a small number of functions in the package; it is likely best practice to invoke these using AnVILPublish::...() rather than attaching the package to the search path.

1.2 The gcloud SDK

It is necessary to have the gcloud SDK available to copy notebook files to the workspace. Test availability with


and verify that the account and project are appropriate (consistent with AnVIL credentials) for use with AnVIL


Note that these be used to set, as well as interrogate, the acount and project.

1.3 Quarto software

Conversion of Rmarkdown (.Rmd) or Quarto (.Qmd) vignettes to Jupyter (.ipynb) notebooks uses Quarto software. It must be available from within R, e.g.,

system2("quarto", "--version")

The user must determine if they want their vignettes converted or rendered into Jupyter notebooks. The difference is that render automatically executes R code blocks and embeds images, while convert will not.

Use of Python notedown for conversion is no longer supported.

2 Creating or updating workspaces

CAUTION updating an existing workspace will replace existing content in a way that cannot be undone – you will lose content!

Workspace creation or update uses information from the DESCRIPTION file, CSV files in inst/tables, and from the YAML metadata at the top of vignettes. It is therefore worth-while to make sure this information is accurate.

In the DESCRIPTION file, the Title, Version, Date, (preferred) or Author / Maintainer fields, Description, and License fields are used.

Tables in inst/tables must be CSV files. Individual entries in the CSV file may contain ‘whisker’ expressions for variable substitution, as follows:

Tables are processed first with whisker.render() for variable substitution, and then readr::read_csv() and avtable_import().Q

In vignettes, the title: and author: name: fields are used. The abstract is a good candidate for future inclusion.

2.1 From package source

The one-stop route is to create a workspace from the package source (e.g., github checkout) directory using as_workspace().

    "bioconductor-rpci-anvil",     # i.e., billing account
    create = TRUE                  # use update = TRUE for an existing workspace

Use create = TRUE to create a new workspace. Use update = TRUE to update (and potentially overwrite) an existing workspace. One of create and update must be TRUE. The command illustrated above does not specify the name = argument, so creates or updates a workspace "Bioconductor-Package-<pkgname>, where <pkgname> is the name of the package read from the DESCRIPTION file; provide an explicit name to create or update an arbitrary workspace. The option use_readme = TRUE appends a file to the formatted content of DESCRIPTION file.

AnVILPublish::as_workspace() invokes as_notebook() so this step does not need to be performed ‘by hand’.

See the command add_access(), below, to make the workspace available to a wider audience.

2.2 From collections of Rmd files

Some R resources, e.g., bookdown sites, are not in packages. These can be processed to workspaces with minor modifications.

  1. Add a standard DESCRIPTION file (e.g., use_this::use_description()) to the directory containing the .Rmd files.

  2. Use the Package: field to provide a one-word identifier (e.g., Package: Bioc2020_CNV) for your material. Add a key-value pair Type: Workshop or similar. The Pacakge: and Type: fields will be used to create the workspace name as, in the example here, Bioconductor-Workshop-Bioc2020_CNV.

  3. Add a ‘yaml’ chunk to the top of each .Rmd file, if not already present, including the title and (optionally) name information, e.g.,

    title: "01. Introduction to the workshop"
    - name: Iman Author
    - name: Imanother Author

Publish the resources with

    "path/to/directory",      # directory containing DESCRIPTION file
    create = TRUE

3 Updating notebooks or workspace permissions

These steps are performed automatically by as_workspace(), but may be useful when developing a new workspace or revising existing workspaces.

3.1 Updating workspace notebooks from vignettes

Transforming vignettes to notebooks may require several iterations, and is available as a separate operation. Use update = FALSE to create local copies for preview.

    "bioconductor-rpci-anvil",     # i.e., billing account
    "Bioconductor-Package-Foo",    # Workspace name
    update = FALSE                 # make notebooks, but do not update workspace

The vignette transformation process has several limitations. Only .Rmd vignettes are supported. Currently, the vignette is transformed first to a markdown document using the rmarkdown command render(..., md_document()). The markdown document is then translated to Python notebook using quarto.

It is likely that some of the limitations of vignette rendering can be reduced.

3.2 Adding user access credentials to share the notebook

The "Bioconductor_User" group can be added to the entities that can see the workspace. AnVIL users wishing to view the workspace should be added to the Bioconductor_User group, rather than to the workspace directly. To add the user group, use


4 Vignette and .Rmd best practices

4.1 Orientation

.Rmd files need to be converted to jupyter notebooks. These ‘best practices’ lead to results that are more likely to be satisfactory, as outlined here.

4.2 Best practices

  1. For packages, make sure the DESCRIPTION file is complete. Use the Authors@R notation for fully specifying authors. Add a Date: field indicating date of last modification. Follow other Bioconductor best practices, e.g., using and incrementing appropriate version numbers.

  2. For collections of vignettes not in a package (e.g., a bookdown folder), add a DESCRIPTION file at the top level. An example is

    Package: BCC2020
    Type: Workshop
    Title: R / Bioconductor in the AnVIL Cloud
    Version: 1.0.0
            given = "Martin",
            family = "Morgan",
            role = c("aut", "cre"),
            email = "",
            comment = c(ORCID = "0000-0002-5874-8148")
        person("Nitesh", "Turaga", role = "ctb"),
        person("Lori", "Shepherd", role = "ctb"))
        This book contains material for a 2 1/2 hour course offered at the
        Bioinformatics Community Conference 2020. Bioconductor provides
        more than 1900 R packages for the analysis and comprehension of
        high-throughput genomic data. Most users install and run
        Bioconductor on a personal computer or perhaps use an academic
        cluster. Cloud-based solutions are increasing appealing, removing
        the headaches of local installation while providing access to (a)
        better, scalable computing resources; and (b) large-scale
        'consortium' and other reference data sets. This session
        introduces the AnVIL cloud computing environment. We cover use of
        the cloud as a replacement to desktop-style computing; integrating
        workflows for 'upstream' processing of large data resources with
        interactive 'downstream' analysis and comprehension, using Human
        Cell Atlas single-cell datasets as an example; and querying
        cloud-based consortium data for integration with a users own data
    License: CC-BY
    Date: 2020-07-17
    Encoding: UTF-8
    LazyData: true
    Roxygen: list(markdown = TRUE)
    RoxygenNote: 7.1.1

    The Type and Package fields are used to construct the second and third elements of the workspace name (in this case, Bioconductor-Workshop-BCC2020). Title, Version, Authors@R, Description, License, and Date fields are used to construct the DASHBOARD page.

  3. Start each vignette with ‘yaml’ containing essential metadata about the document – title and author(s). Include other information if desired, e.g., abstract, (static) date of last modification.

  4. Use a file naming system AND a yaml title field that sorts files into the order in which the document content is to be presented, e.g., using file names 01-Setup.Rmd, 02-... and titles (in the yaml) title: "01 Setup", … Naming both files and titles in this way provides some chance that the Rmd files are presented, or can be made to be presented, sensibly across the Bioconductor package landing page and Workspace / NOTEBOOK interface.

  5. All code chunks, regardless of annotations such as eval = FALSE or echo = FALSE are converted to visible, evaluated cells in jupyter notebooks. Replace code chunks that you do not wish the user to evaluate with HTML tags <pre></pre>.

  6. Although both Rmarkdown and python notebooks support code chunks in multiple languages, there is no support for this in the conversion process – all cells are presented as R code.

4.3 Additional notes on .Rmd conversion

Current best practice is to use quarto for conversion of .Rmd to ipynb. Quarto is available on the Bioconductor docker image, or easily installed on Linux, macOS, or Windows.

Support for conversion using the Python notedown module is no longer supported.

Session info

## R version 4.3.1 (2023-06-16)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 22.04.3 LTS
## Matrix products: default
## BLAS:   /home/biocbuild/bbs-3.18-bioc/R/lib/ 
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_GB              LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## time zone: America/New_York
## tzcode source: system (glibc)
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## other attached packages:
## [1] BiocStyle_2.30.0
## loaded via a namespace (and not attached):
##  [1] digest_0.6.33       R6_2.5.1            bookdown_0.36      
##  [4] fastmap_1.1.1       xfun_0.40           cachem_1.0.8       
##  [7] knitr_1.44          htmltools_0.5.6.1   rmarkdown_2.25     
## [10] cli_3.6.1           sass_0.4.7          jquerylib_0.1.4    
## [13] compiler_4.3.1      tools_4.3.1         evaluate_0.22      
## [16] bslib_0.5.1         yaml_2.3.7          BiocManager_1.30.22
## [19] jsonlite_1.8.7      rlang_1.1.1