The epimutacionsData package is a repository of datasets for the
epimutacions package. It includes 2 datasets to use as an example:
The following code explains how to access to the data:
library(ExperimentHub)
eh <- ExperimentHub()
query(eh, c("epimutacionsData"))## ExperimentHub with 3 records
## # snapshotDate(): 2023-10-24
## # $dataprovider: GEO, Illumina 450k array
## # $species: Homo sapiens
## # $rdataclass: RGChannelSet, GenomicRatioSet, GRanges
## # additional mcols(): taxonomyid, genome, description,
## # coordinate_1_based, maintainer, rdatadateadded, preparerclass, tags,
## # rdatapath, sourceurl, sourcetype
## # retrieve records with, e.g., 'object[["EH6690"]]'
##
## title
## EH6690 | Control and case samples
## EH6691 | Reference panel
## EH6692 | Candidate epimutationsIn Illumina 450K array (Reproducibility 2012), probes are unequally distributed along the genome, limiting the number of regions that can fulfil the requirements to be considered an epimutation. So, we have computed a dataset containing the regions that are candidates to become an epimutation.
To define the candidate epimutations,
we relied on the clustering from bumphunter (Jaffe et al. 2012).
We defined a primary dataset with all the CpGs from the Illumina 450K array.
Then, we run bumphunter and selected those regions with at least 3 CpGs.
As a result, we found 40408 candidate epimutations
which are available in the candRegsGR dataset.
candRegsGR <- eh[["EH6692"]]The package includes an RGChannelSet class reference panel
(reference_panel)
which contains 22 whole cord blood samples from
healthy children born via caesarian from
the GSE127824 cohort (Gervin et al. 2019).
The reference panel can be found in EH6691 record of the eh object:
reference_panel <- eh[["EH6691"]]The IDAT files contain raw microarray intensities of 4 case samples
from GSE131350 cohort.
The files are located on the external data of epimutacionsData package:
library(minfi)
baseDir <- system.file("extdata", package = "epimutacionsData")
targets <- read.metharray.sheet(baseDir)sessionInfo()## R Under development (unstable) (2023-10-22 r85388)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 22.04.3 LTS
##
## Matrix products: default
## BLAS: /home/biocbuild/bbs-3.19-bioc/R/lib/libRblas.so
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.10.0
##
## locale:
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## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
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## time zone: America/New_York
## tzcode source: system (glibc)
##
## attached base packages:
## [1] parallel stats4 stats graphics grDevices utils datasets
## [8] methods base
##
## other attached packages:
## [1] minfi_1.49.0 bumphunter_1.45.0
## [3] locfit_1.5-9.8 iterators_1.0.14
## [5] foreach_1.5.2 Biostrings_2.71.1
## [7] XVector_0.43.0 SummarizedExperiment_1.33.0
## [9] Biobase_2.63.0 MatrixGenerics_1.15.0
## [11] matrixStats_1.0.0 GenomicRanges_1.55.1
## [13] GenomeInfoDb_1.39.0 IRanges_2.37.0
## [15] S4Vectors_0.41.1 epimutacionsData_1.7.0
## [17] ExperimentHub_2.11.0 AnnotationHub_3.11.0
## [19] BiocFileCache_2.11.1 dbplyr_2.4.0
## [21] BiocGenerics_0.49.0 BiocStyle_2.31.0
##
## loaded via a namespace (and not attached):
## [1] RColorBrewer_1.1-3 jsonlite_1.8.7
## [3] magrittr_2.0.3 GenomicFeatures_1.55.1
## [5] rmarkdown_2.25 BiocIO_1.13.0
## [7] zlibbioc_1.49.0 vctrs_0.6.4
## [9] multtest_2.59.0 memoise_2.0.1
## [11] Rsamtools_2.19.0 DelayedMatrixStats_1.25.0
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## [31] Matrix_1.6-1.1 R6_2.5.1
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## [51] BiocParallel_1.37.0 DBI_1.1.3
## [53] HDF5Array_1.31.0 biomaRt_2.59.0
## [55] MASS_7.3-60.1 openssl_2.1.1
## [57] rappdirs_0.3.3 DelayedArray_0.29.0
## [59] rjson_0.2.21 tools_4.4.0
## [61] interactiveDisplayBase_1.41.0 httpuv_1.6.12
## [63] quadprog_1.5-8 glue_1.6.2
## [65] restfulr_0.0.15 nlme_3.1-163
## [67] rhdf5filters_1.15.0 promises_1.2.1
## [69] grid_4.4.0 generics_0.1.3
## [71] tzdb_0.4.0 preprocessCore_1.65.0
## [73] tidyr_1.3.0 data.table_1.14.8
## [75] hms_1.1.3 xml2_1.3.5
## [77] utf8_1.2.4 BiocVersion_3.19.1
## [79] pillar_1.9.0 stringr_1.5.0
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## [93] knitr_1.45 bookdown_0.36
## [95] xfun_0.40 scrime_1.3.5
## [97] statmod_1.5.0 stringi_1.7.12
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## [101] codetools_0.2-19 tibble_3.2.1
## [103] BiocManager_1.30.22 cli_3.6.1
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## [119] purrr_1.0.2 crayon_1.5.2
## [121] rlang_1.1.1 KEGGREST_1.43.0Butcher, Darci T, Cheryl Cytrynbaum, Andrei L Turinsky, Michelle T Siu, Michal Inbar-Feigenberg, Roberto Mendoza-Londono, David Chitayat, et al. 2017. “CHARGE and Kabuki Syndromes: Gene-Specific Dna Methylation Signatures Identify Epigenetic Mechanisms Linking These Clinically Overlapping Conditions.” The American Journal of Human Genetics 100 (5): 773–88.
Gervin, Kristina, Lucas A Salas, Kelly M Bakulski, Menno C Van Zelm, Devin C Koestler, John K Wiencke, Liesbeth Duijts, et al. 2019. “Systematic Evaluation and Validation of Reference and Library Selection Methods for Deconvolution of Cord Blood Dna Methylation Data.” Clinical Epigenetics 11 (1): 1–15.
Jaffe, Andrew E, Peter Murakami, Hwajin Lee, Jeffrey T Leek, M Daniele Fallin, Andrew P Feinberg, and Rafael A Irizarry. 2012. “Bump Hunting to Identify Differentially Methylated Regions in Epigenetic Epidemiology Studies.” International Journal of Epidemiology 41 (1): 200–209.
Reproducibility, Unrivaled Assay. 2012. “Infinium Humanmethylation450 Beadchip.”
Shi, Lei, Fan Jiang, Fengxiu Ouyang, Jun Zhang, Zhimin Wang, and Xiaoming Shen. 2018. “DNA Methylation Markers in Combination with Skeletal and Dental Ages to Improve Age Estimation in Children.” Forensic Science International: Genetics 33: 1–9.