As the number of cells that are routinely interrogated in single-cell experiments increases rapidly and can easily reach hundreds of thousands, the computational resource requirements also grow larger. Several approaches have been proposed to either subsample or aggregate cells in order to reduce the size of the data and enable the application of standard analysis procedures. One such approach is geometric sketching - subsampling in a density-aware manner in such a way that densely populated regions of the gene expression space are subsampled more aggressively, while a larger fraction of cells are retained in sparsely populated regions. In addition to reducing the size of the data set, this often also increases the relative representation of rare cell types in the subsampled data set.
Several tools have been developed for applying sketching to single-cell (or other) data sets, but not all of them are easily applicable from R. The sketchR
package implements an R/Bioconductor interface to some of the most popular python packages for geometric sketching, allowing them to be directly incorporated into Bioconductor-based single-cell analysis workflows. The interaction with python is managed using the basilisk
package, which automatically takes care of generating and activating a suitable conda environment with the required packages.
This vignette showcases the main functionalities of the sketchR
package, and illustrates how it can be used to generate a subsample of a dataset using the geometric sketching/subsampling algorithms and implementations proposed by Hie et al. (2019) and Song et al. (2022), as well as create a set of diagnostic plots.
sketchR
can be installed from Bioconductor using the following code:
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("sketchR")
We start by loading the required packages and preparing an example data set.
suppressPackageStartupMessages({
library(sketchR)
library(TENxPBMCData)
library(scuttle)
library(scran)
library(scater)
library(SingleR)
library(celldex)
library(cowplot)
library(SummarizedExperiment)
library(SingleCellExperiment)
library(beachmat.hdf5)
})
We will use the PBMC3k data set from the TENxPBMCData Bioconductor package for illustration. The chunk below prepares the data set by calculating log-transformed normalized counts, finding highly variable genes, performing dimensionality reduction and predicting cell type labels using the SingleR package.
## Load data
pbmc3k <- TENxPBMCData::TENxPBMCData(dataset = "pbmc3k")
#> see ?TENxPBMCData and browseVignettes('TENxPBMCData') for documentation
#> loading from cache
## Set row and column names
colnames(pbmc3k) <- paste0("Cell", seq_len(ncol(pbmc3k)))
rownames(pbmc3k) <- scuttle::uniquifyFeatureNames(
ID = SummarizedExperiment::rowData(pbmc3k)$ENSEMBL_ID,
names = SummarizedExperiment::rowData(pbmc3k)$Symbol_TENx
)
## Normalize and log-transform counts
pbmc3k <- scuttle::logNormCounts(pbmc3k)
## Find highly variable genes
dec <- scran::modelGeneVar(pbmc3k)
top.hvgs <- scran::getTopHVGs(dec, n = 2000)
## Perform dimensionality reduction
set.seed(100)
pbmc3k <- scater::runPCA(pbmc3k, subset_row = top.hvgs)
pbmc3k <- scater::runTSNE(pbmc3k, dimred = "PCA")
## Predict cell type labels
ref_monaco <- celldex::MonacoImmuneData()
pred_monaco_main <- SingleR::SingleR(test = pbmc3k, ref = ref_monaco,
labels = ref_monaco$label.main)
pbmc3k$labels_main <- pred_monaco_main$labels
dim(pbmc3k)
#> [1] 32738 2700
The geosketch()
function performs geometric sketching by calling the geosketch
python package. The output is a vector of indices that can be used to subset the full dataset. The provided seed will be propagated to the python code to achieve reproducibility.
idx800gs <- geosketch(SingleCellExperiment::reducedDim(pbmc3k, "PCA"),
N = 800, seed = 123)
#> + /media/volume/teran2_disk/biocbuild/.cache/R/basilisk/1.17.2/0/bin/conda create --yes --prefix /media/volume/teran2_disk/biocbuild/.cache/R/basilisk/1.17.2/sketchR/1.1.3/universal 'python=3.11.10' --quiet -c bioconda -c conda-forge --override-channels
#> + /media/volume/teran2_disk/biocbuild/.cache/R/basilisk/1.17.2/0/bin/conda install --yes --prefix /media/volume/teran2_disk/biocbuild/.cache/R/basilisk/1.17.2/sketchR/1.1.3/universal 'python=3.11.10' -c bioconda -c conda-forge --override-channels
#> + /media/volume/teran2_disk/biocbuild/.cache/R/basilisk/1.17.2/0/bin/conda install --yes --prefix /media/volume/teran2_disk/biocbuild/.cache/R/basilisk/1.17.2/sketchR/1.1.3/universal -c bioconda -c conda-forge 'python=3.11.10' '_libgcc_mutex=0.1' '_openmp_mutex=4.5' 'anndata=0.10.9' 'array-api-compat=1.9' 'brotli=1.1.0' 'brotli-bin=1.1.0' 'bzip2=1.0.8' 'c-ares=1.33.1' 'ca-certificates=2024.8.30' 'cached-property=1.5.2' 'cached_property=1.5.2' 'certifi=2024.8.30' 'colorama=0.4.6' 'contourpy=1.3.0' 'cycler=0.12.1' 'exceptiongroup=1.2.2' 'fonttools=4.54.1' 'freetype=2.12.1' 'get-annotations=0.1.2' 'h5py=3.11.0' 'hdf5=1.14.3' 'icu=75.1' 'joblib=1.4.2' 'keyutils=1.6.1' 'kiwisolver=1.4.7' 'krb5=1.21.3' 'lcms2=2.15' 'ld_impl_linux-64=2.43' 'legacy-api-wrap=1.4' 'lerc=4.0.0' 'libaec=1.1.3' 'libblas=3.9.0' 'libbrotlicommon=1.1.0' 'libbrotlidec=1.1.0' 'libbrotlienc=1.1.0' 'libcblas=3.9.0' 'libcurl=8.10.1' 'libdeflate=1.18' 'libedit=3.1.20191231' 'libev=4.33' 'libexpat=2.6.3' 'libffi=3.4.2' 'libgcc=14.1.0' 'libgcc-ng=14.1.0' 'libgfortran=14.1.0' 'libgfortran-ng=14.1.0' 'libgfortran5=14.1.0' 'libgomp=14.1.0' 'libhwloc=2.11.1' 'libiconv=1.17' 'libjpeg-turbo=2.1.5.1' 'liblapack=3.9.0' 'libllvm14=14.0.6' 'libnghttp2=1.58.0' 'libnsl=2.0.1' 'libopenblas=0.3.27' 'libpng=1.6.44' 'libsqlite=3.46.1' 'libssh2=1.11.0' 'libstdcxx=14.1.0' 'libstdcxx-ng=14.1.0' 'libtiff=4.5.1' 'libuuid=2.38.1' 'libwebp-base=1.4.0' 'libxcb=1.15' 'libxcrypt=4.4.36' 'libxml2=2.12.7' 'libzlib=1.3.1' 'llvmlite=0.43.0' 'matplotlib-base=3.9.2' 'munkres=1.1.4' 'natsort=8.4.0' 'ncurses=6.5' 'networkx=3.3' 'numba=0.60.0' 'numpy=1.26.4' 'openjpeg=2.5.0' 'openssl=3.3.2' 'packaging=24.1' 'pandas=2.2.3' 'patsy=0.5.6' 'pillow=10.0.0' 'pip=24.2' 'pthread-stubs=0.4' 'pynndescent=0.5.13' 'pyparsing=3.1.4' 'python=3.11.10' 'python-dateutil=2.9.0' 'python-tzdata=2024.2' 'python_abi=3.11' 'pytz=2024.1' 'qhull=2020.2' 'readline=8.2' 'scanpy=1.10.3' 'scikit-learn=1.5.2' 'scipy=1.13.1' 'seaborn=0.13.2' 'seaborn-base=0.13.2' 'session-info=1.0.0' 'setuptools=75.1.0' 'six=1.16.0' 'statsmodels=0.14.4' 'stdlib-list=0.10.0' 'tbb=2021.13.0' 'threadpoolctl=3.5.0' 'tk=8.6.13' 'tqdm=4.66.5' 'tzdata=2024b' 'umap-learn=0.5.6' 'wheel=0.44.0' 'xorg-libxau=1.0.11' 'xorg-libxdmcp=1.1.5' 'xz=5.2.6' 'zstd=1.5.6' --override-channels
head(idx800gs)
#> [1] 3 6 9 11 15 16
length(idx800gs)
#> [1] 800
Similarly, the scsampler()
function calls the scSampler
python package to perform subsampling.
idx800scs <- scsampler(SingleCellExperiment::reducedDim(pbmc3k, "PCA"),
N = 800, seed = 123)
head(idx800scs)
#> [1] 1079 644 1494 1278 5 391
length(idx800scs)
#> [1] 800
To illustrate the result of the subsampling, we plot the tSNE representation of the original data as well as the two subsets (using the tSNE coordinates derived from the full dataset).
cowplot::plot_grid(
scater::plotTSNE(pbmc3k, colour_by = "labels_main"),
scater::plotTSNE(pbmc3k[, idx800gs], colour_by = "labels_main"),
scater::plotTSNE(pbmc3k[, idx800scs], colour_by = "labels_main")
)