Contents

1 Launch the Shiny APP

2 Loading .RData to Shiny APP

After the analysis was done using cytofkit, a special RData file will be saved with suffix of .RData. This RData file is saved for loading to the shiny APP to explore the results (The RData file used in this vignette can be found on the github ).

Click the top-left Choose File button to choose RData file, then click the submit button below to load the data.

For use on servers, the Server File Select button can be used to load data directly from server without storing it locally.

As shown in the figure above, there are one side panel and one main panel for the cytofkit shiny APP.

3 Cluster Panel

Inside the cluster panel, there are three tab panels, Cluster Plot, Annotate Clusters and Run FlowSOM.